What is Structured Illumination Microscopy? Structured Illumination Microscopy (SIM) is a super-resolution fluorescence microscopy technique that allows for imaging beyond the diffraction limit of ...

Structured illumination microscopy (SIM) is the most preferable system for live-cell super-resolution imaging. It enables the observation of intricate subcellular dynamics. However, conventional SIM ...

Confocal microscopy is a specialized fluorescence imaging technique that scientists use to acquire images at greater resolution than conventional microscopy. 1 In addition to scanning the lateral x ...

Top: Random Illumination Microscopy (RIM) utilizes random speckle patterns to achieve super-resolution imaging of biological tissues, improving resolution by a factor of two compared to the optical ...

For hundreds of years, the optical microscope was the only tool available to scientists wanting to study the movement of cells, bacteria and yeast. But the diffraction of light made it impossible to ...

SIMIP enables high-resolution images rich in both chemical and spatial information. A quantum cascade laser (QCL) excites molecular vibrations while a spatial light modulator (SLM) generates striped ...

Researchers have incorporated a swept illumination source into an open-top light-sheet microscope to enable improved optical sectioning over a larger area of view. The advance makes the technique more ...

A project at Swiss research center EPFL has designed an add-on unit intended to bring structured light capability to a standard optical microscope. Described in Nature Communications, the design ...

QIScope: When imaging low protein levels in live cells on the high-sensitivity QIScope, bioluminescence (blue) significantly outperforms fluorescence (green). (Courtesy: Ruyu Ma - Helmholtz Munich) A ...