With the technique called polymerase chain reaction (PCR), scientists can make multiple copies of a specific genetic sequence within DNA. PCR is a powerful tool for researchers because it allows for ...

Scientists convened in Munich last month for the “International qPCR Symposium” to discuss what the event’s organizers described as “the most powerful analytical technology ever developed in the life ...

While many researchers planning PCRs carefully select their DNA polymerase and buffer, they often neglect the other reaction components that play a large role in PCR success. From the template and ...

Quantitative real-time PCR (qPCR) and digital PCR (dPCR) are powerful molecular biology techniques for quantifying and analyzing DNA or RNA, but they differ in their approach and applications.

Individual components, including the template, primers, polymerase, dNTP levels, and cycling conditions, can impact the overall PCR reaction. Explore how these ...

What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...