T cells play an important role in the immune system. Below, you can see such a t cell expressing Lifeact-GFP. The video shows a color-coded depth projection and a maximum intensity projection side-by-side. The T cell was imaged constantly for over 1 hour; one volume every 2.5 seconds. Sample: courtesy of M. Fritzsche, University of Oxford, UK. Acquired with ZEISS Lattice Lightsheet 7: https://zeiss.ly/fb-lattice-lightsheet-7 #zeiss #microscopy #tcell #tcells #latticelightsheet7 | ZEISS Microscop
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T cells play an important role in the immune system. Below, you can s…
1.2K viewsJan 19, 2021
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An engineered T cell (green) seeking out and destroying a cancerous target cell (magenta). The movie shows transient calcium fluxes in the T cell prior to the destruction of the target which lights up with PI upon death. It has been imaged using lattice light sheet microscopy (ZEISS Lattice Lightsheet 7) at the Centre for Dynamic Imaging (CDI) at WEHI - The Walter and Eliza Hall Institute in Parkville, Australia 🇦🇺. The Centre is both a core facility and a research lab for advanced microscopy.
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An engineered T cell (green) seeking out and destroying a cancerous ta…
608 viewsMar 28, 2022
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Observing life. COS-7 cell expressing Lifeact-GFP. Maximum intensity projection. The cell was imaged constantly for 9 hours using ZEISS Lattice Lightsheet 7; one volume (115 × 60 × 25 µm³) every 10 seconds. A total of 1.005.000 images was recorded; 201 volume planes for 5000 time points. Learn more about ZEISS Lattice Lightsheet 7: https://zeiss.ly/fb-lattice-lightsheet-7 | ZEISS Microscopy
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Observing life. COS-7 cell expressing Lifeact-GFP. Maximu…
1.8K viewsFeb 16, 2021
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The video below shows the imaging of extremely delicate and light-sensitive organelles of a COS-7 cell. It was transiently transfected with Tomm20-mEmerald and Calreticulin-tdTomato. Tomm20 labels the outer membrane of mitochondria, Calreticulin is a protein of the endoplasmic reticulum (ER), where proteins are synthesized. Both are difficult to image with conventional methods. The example shows ER wrapping around mitochondria and assisting mitochondrial fission. -------------- One volume every
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The video below shows the imaging of extremely delicate and light-sen…
623 viewsJun 22, 2022
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A time lapse movie showing the dynamics of a U2OS cell. Its cytoskeleton is labeled in cyan (Actin-GFP), mitochondria in green (MitoTracker™ Red CMXRos), and its nucleus in magenta (Draq 5). Imaged with ZEISS Lattice Lightsheet 7: https://zeiss.ly/fb-lattice-lightsheet-7 | ZEISS Microscopy
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A time lapse movie showing the dynamics of a U2OS cell. Its cytos…
452 viewsOct 8, 2021
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